| # | Athlete | Team | Seed | |
|---|---|---|---|---|
| 1 | 335 | KEREBEL, Nicolas | Unattached Ontario | 54.00 |
| 2 | 278 | CRAWFORD, James | Kitchener Waterloo T.F.A. | 55.09 |
| 3 | 285 | DELSAUT, Frank | Kitchener Waterloo T.F.A. | 56.02 |
| 4 | 330 | JOHNSON, Dylan | London Western T.F.C. | 57.24 |
| 5 | 262 | BRUGER, Harrison | Eclipse Track & Field Inc | 1:13.00 |
| 6 | 277 | CRAWFORD, Calum | Kitchener Waterloo T.F.A. | NT |
| 7 | 314 | HAMDY, Gabe | Eclipse Track & Field Inc | NT |
| 8 | 354 | MCKELVIE, Lochlan | Eclipse Track & Field Inc | NT |
| 9 | 377 | ROBINSON, Haiden | Sarnia Athletics Southwest TFC | NT |